Category: Blogging

Sheep Plasma K3EDTA (Unfl) — Research-Grade Matrix, Chelation Chemistry, and Pre-Analytical Controls

What is Sheep Plasma K3EDTA (Unfl)? Sheep plasma K3EDTA (unfiltered) is whole plasma collected from ovine blood into tubes containing tripotassium ethylenediaminetetraacetic acid (K₃EDTA) as anticoagulant, with no post-draw membrane/0.2 µm/0.1 µm filtration step. EDTA acts by chelating Ca²⁺, preventing coagulation...

DNA PCR Quantitative Positive Control for qPCR & dPCR—Copy-Number Standards, MIQE-style Reporting, Uncertainty, and Inhibition Checks

Scope and Intent (Research Only) This article describes design, qualification, and routine use of a DNA PCR quantitative positive control for real-time PCR (qPCR) and digital PCR (dPCR) in research workflows. It focuses on copy-number traceability, run qualification, instrument comparability, and...