Inappropriate use of antimicrobial brokers in animal manufacturing has led to the event of antimicrobial resistance (AMR) in foodborne pathogens. Transmission of AMR foodborne pathogens from reservoirs, notably chickens to the human inhabitants does happen. Recently, we reported that occupational publicity was a threat issue for multidrug-resistant (MDR) Escherichia coli (E. coli) amongst poultry-workers. Here we decided the prevalence and genetic relatedness amongst MDR E. coli isolated from poultry-workers, chickens, and poultry environments in Abuja, Nigeria. This examine was carried out to handle the gaps recognized by the Nigerian AMR scenario evaluation.
We carried out a cross-sectional examine amongst poultry-workers, chickens, and poultry farm/reside chook market (LBM) environments. The isolates had been examined phenotypically for his or her antimicrobial susceptibility profiles, genotypically characterised utilizing whole-genome sequencing (WGS) and in silico multilocus sequence sorts (MLST). We carried out a phylogenetic single nucleotide polymorphism (SNPs) evaluation to find out relatedness and clonality among the many isolates. Two plasmid-mediated colistin genes-mcr-1.1 harboured on IncX4 plasmids had been detected in environmental isolates.
A complete of 115 (26.8%) out of 429 samples had been optimistic for E. coli. Of these, 110 isolates had been viable for phenotypic and genotypic characterization. The choice comprised 47 (42.7%) isolates from poultry-workers, 36 (32.7%) from chickens, and 27 (24.5%) from poultry-farm or LBM environments. Overall, 101 (91.8%) of the isolates had been MDR conferring resistance to not less than three drug lessons. The most prevalent sequence sorts (ST) had been ST-155 (n = 8), ST-48 (n = 8) and ST-10 (n = 6). Two isolates of human and environmental sources with a SNPs distinction of 6161 originating from the identical farm shared a novel ST.
MDR E.coli isolates had been prevalent amongst poultry-workers, poultry, and the poultry farm/LBM surroundings. The emergence of MDR E. coli with novel ST in two isolates could also be plasmid-mediated. Competent authorities ought to implement AMR laws to make sure prudent use of antimicrobials to restrict the chance of transmission alongside the meals chain. High frequency of resistance was noticed for tetracycline (n = 102; 92.7%), trimethoprim/sulfamethoxazole (n = 93; 84.5%), streptomycin (n = 87; 79.1%) and ampicillin (n = 88; 80%). The isolates had related AMR genes and plasmid replicons.
Role of the rooster oligoadenylate synthase-like gene throughout in vitro Newcastle illness virus an infection
The enzyme 2′-5′ oligoadenylate synthase (OAS) is one of the important thing interferon-induced antiviral elements that act by inhibition of viral replication. In chickens, there’s a single well-characterized OAS gene, oligoadenylate synthase-like (OASL) that has been proven to be upregulated after an infection with varied viruses. However, a deeper understanding of how rooster OASL acts towards viral an infection remains to be essential. In this examine, we examined the speculation that OASL brief interfering RNA (siRNA)-mediated knockdown would lower the host gene expression response to the Newcastle illness virus (NDV) by impacting antiviral pathways. To assess our speculation, a rooster fibroblast cell line (DF-1) was contaminated with the NDV (LaSota pressure) and OASL expression was knocked down utilizing a selected siRNA.
The stage of NDV viral RNA within the cells and the expression of interferon response- and apoptosis-related genes had been evaluated by quantitative PCR at 4, 8, and 24 h postinfection (hpi). Knockdown of OASL elevated the extent of NDV viral RNA at 4, 8, and 24 hpi (P < 0.05) and eradicated the distinction between NDV-infected and noninfected cells for expression of interferon response- and apoptosis-related genes (P > 0.05). The lack of differential expression means that knockdown of OASL resulted in a decreased response to NDV an infection. Within NDV-infected cells, OASL knockdown decreased expression of sign transducer and activator of transcription 1, interferon alfa receptor subunit 1
eukaryotic translation initiation issue 2 alpha kinase 2, ribonuclease L, caspase 8 (CASP8) and caspase 9 (CASP9) at Four hpi, CASP9 at Eight hpi, and caspase 3, CASP8, and CASP9 at 24 hpi (P < 0.05). We recommend that the elevated NDV viral load in DF-1 cells after OASL knockdown was the outcome of a fancy interplay between OASL and interferon response- and apoptosis-related genes that decreased host response to the NDV. Our outcomes present complete data on the function performed by OASL throughout NDV an infection in vitro. Targeting this mechanism may assist in future prophylactic and therapeutic therapies for Newcastle illness in poultry.
Chickens productiveness choice impacts immune system genes
Analysis of the protein-coding sequences of the CD1B and DMA genes of varied breeds of chickens revealed a detrimental choice of these genes. In order to know whether or not the fixation of the studied alleles is the outcome of synthetic choice of the B5 line of the cross Smena 8, an evaluation of related loci in layer chickens Hisex White was carried out. The frequencies of the alleles on the loci of the CD1B gene and the RACK1 gene within the Hisex White rooster genome differ from the frequencies of the alleles obtained for chickens of the B5 line of the cross Smena 8. It may be assumed that the fixation of the allele within the DMA gene is related to synthetic or pure choice, constant in broilers and layers.
 Human Stomach PrimaCell: Normal Stomach Mucosa Epithelial Cells |
|
2-96104 |
CHI Scientific |
1 Kit |
Ask for price |
 Chicken Actin, cytoplasmic 1, ACTB ELISA KIT |
|
ELI-34873c |
Lifescience Market |
96 Tests |
EUR 928 |
 Chicken Cytoplasmic aconitate hydratase, ACO1 ELISA KIT |
|
ELI-49460c |
Lifescience Market |
96 Tests |
EUR 928 |
 Chicken Actin, cytoplasmic 2, ACTG1 ELISA KIT |
|
ELI-24524c |
Lifescience Market |
96 Tests |
EUR 928 |
 Chicken Aspartate aminotransferase, cytoplasmic, GOT1 ELISA KIT |
|
ELI-04025c |
Lifescience Market |
96 Tests |
EUR 928 |
 Chicken Malate dehydrogenase, cytoplasmic, MDH1 ELISA KIT |
|
ELI-06826c |
Lifescience Market |
96 Tests |
EUR 928 |
 Stomach Tumor Lysate |
|
1340 |
ProSci |
0.1 mg |
EUR 254 |
|
Description: Stomach tumor tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
 Stomach-Pylorus Lysate |
|
21-325 |
ProSci |
0.1 mg |
EUR 390.5 |
|
Description: Monkey (Rhesus) stomach-pylorus tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Rhesus) stomach-pylorus tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the stomach-pylorus tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The stomach-pylorus tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
 Fetal Stomach Lysate |
|
XBL-10423 |
ProSci |
0.1 mg |
EUR 416.75 |
|
Description: Fetal human stomach tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The fetal human stomach tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the stomach tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The stomach tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
 Stomach Membrane Lysate |
|
XBL-10924 |
ProSci |
0.1 mg |
EUR 516.5 |
|
Description: Human stomach tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human stomach tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated stomach tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated stomach tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal. |
 ELISA Kit) Chicken Hydroxymethylglutaryl-CoA Synthase, Cytoplasmic (HMGCS1) ELISA Kit |
|
abx518370-96tests |
Abbexa |
96 tests |
EUR 911 |
|
|
 ELISA Kit) Chicken Threonyl tRNA Synthetase, cytoplasmic (TARS) ELISA Kit |
|
abx356084-96tests |
Abbexa |
96 tests |
EUR 825 |
|
|
) ELISA kit for Chicken Malate dehydrogenase, cytoplasmic (MDH1) |
|
KTE30117-48T |
Abbkine |
48T |
EUR 354 |
|
|
|
Description: Quantitative sandwich ELISA for measuring Chicken Malate dehydrogenase, cytoplasmic (MDH1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Chicken Malate dehydrogenase, cytoplasmic (MDH1) |
|
KTE30117-5platesof96wells |
Abbkine |
5 plates of 96 wells |
EUR 2252 |
|
|
|
Description: Quantitative sandwich ELISA for measuring Chicken Malate dehydrogenase, cytoplasmic (MDH1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Chicken Malate dehydrogenase, cytoplasmic (MDH1) |
|
KTE30117-96T |
Abbkine |
96T |
EUR 572 |
|
|
|
Description: Quantitative sandwich ELISA for measuring Chicken Malate dehydrogenase, cytoplasmic (MDH1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
 Chicken Tyrosyl- tRNA synthetase, cytoplasmic, YARS ELISA KIT |
|
ELI-52138c |
Lifescience Market |
96 Tests |
EUR 928 |
 Chicken Arginyl- tRNA synthetase, cytoplasmic, RARS ELISA KIT |
|
ELI-13682c |
Lifescience Market |
96 Tests |
EUR 928 |
 Chicken Cysteinyl- tRNA synthetase, cytoplasmic, CARS ELISA KIT |
|
ELI-18547c |
Lifescience Market |
96 Tests |
EUR 928 |
 Rat Stomach PrimaCell: Normal Stomach Mucosa Epithelial Cells Growth Medium |
|
9-25085 |
CHI Scientific |
5 x 100 ml |
Ask for price |
 Mouse Stomach PrimaCell: Normal Stomach Mucosa Epithelial Cells Growth Medium |
|
9-32088 |
CHI Scientific |
5 x 100 ml |
Ask for price |
 Human Stomach PrimaCell: Normal Stomach Mucosa Epithelial Cells Growth Medium |
|
9-46104 |
CHI Scientific |
5 x 100 ml |
Ask for price |
 Human Stomach Tissue Preparation Buffer: Normal Stomach Mucosa Epithelial Cells |
|
9-80104 |
CHI Scientific |
1 x 100 ml |
Ask for price |
 Mouse Stomach Tissue Preparation Buffer: Normal Stomach Mucosa Epithelial Cells |
|
9-80202 |
CHI Scientific |
1 x 100 ml |
Ask for price |
 Rat Stomach Tissue Preparation Buffer: Normal Stomach Mucosa Epithelial Cells |
|
9-80292 |
CHI Scientific |
1 x 100 ml |
Ask for price |
 cDNA from Lupus: stomach |
|
C1236248Lup |
Biochain |
40 reactions |
EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Human Stomach Tissue Lysate |
|
30R-AS039 |
Fitzgerald |
150 ug |
EUR 219 |
|
Description: Fresh tissue lysate isolated from human stomach |
) Stomach Tissue Slide (Normal) |
|
10-801-10um |
ProSci |
10 um |
EUR 201.5 |
Stomach Tissue Slide (Normal) |
|
10-801-4um |
ProSci |
4 um |
EUR 180.5 |
) Stomach Tissue Slide (Benign) |
|
10-803-10um |
ProSci |
10 um |
EUR 201.5 |
Stomach Tissue Slide (Benign) |
|
10-803-4um |
ProSci |
4 um |
EUR 180.5 |
) Stomach Tissue Slide (Adenocarcinoma) |
|
10-809-10um |
ProSci |
10 um |
EUR 201.5 |
Stomach Tissue Slide (Adenocarcinoma) |
|
10-809-4um |
ProSci |
4 um |
EUR 180.5 |
) Stomach Tissue Slide (Carcinoma) |
|
10-814-10um |
ProSci |
10 um |
EUR 201.5 |
Stomach Tissue Slide (Carcinoma) |
|
10-814-4um |
ProSci |
4 um |
EUR 180.5 |
) Stomach Tissue Slide (Abnormal) |
|
10-826-10um |
ProSci |
10 um |
EUR 201.5 |
Stomach Tissue Slide (Abnormal) |
|
10-826-4um |
ProSci |
4 um |
EUR 180.5 |
) Stomach Tissue Slide (Tumor) |
|
10-827-10um |
ProSci |
10 um |
EUR 201.5 |
Stomach Tissue Slide (Tumor) |
|
10-827-4um |
ProSci |
4 um |
EUR 180.5 |
) Stomach Tissue Lysate (Normal) |
|
1717-01 |
ProSci |
0.1 mg |
EUR 217.25 |
|
Description: Stomach tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
 Stomach Diabetic Disease Lysate |
|
XBL-10387 |
ProSci |
0.1 mg |
EUR 663.5 |
|
Description: Human stomach tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human stomach tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the stomach tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The stomach tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
 Stomach Membrane Tumor Lysate |
|
XBL-10936 |
ProSci |
0.1 mg |
EUR 626.75 |
|
Description: Human stomach tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human stomach tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated stomach tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated stomach tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal. |
 Stomach-Cardia Membrane Lysate |
|
XBL-10939 |
ProSci |
0.1 mg |
EUR 516.5 |
|
Description: Human stomach: Cardia tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human stomach: Cardia tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated stomach: Cardia tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated stomach: Cardia tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal. |
 Stomach-Corpus Membrane Lysate |
|
XBL-10942 |
ProSci |
0.1 mg |
EUR 516.5 |
|
Description: Human stomach: corpus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human stomach: corpus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated stomach: corpus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated stomach: corpus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal. |
 Stomach-Fundus Membrane Lysate |
|
XBL-10945 |
ProSci |
0.1 mg |
EUR 516.5 |
|
Description: Human stomach: fundus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human stomach: fundus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated stomach: fundus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated stomach: fundus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal. |
 Stomach-Pylorus Membrane Lysate |
|
XBL-10948 |
ProSci |
0.1 mg |
EUR 516.5 |
|
Description: Human stomach: Pylorus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human stomach: Pylorus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated stomach: Pylorus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated stomach: Pylorus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal. |
 ACTB ELISA Kit| chicken Actin, cytoplasmic 1 ELISA Kit |
|
EF012185 |
Lifescience Market |
96 Tests |
EUR 689 |
 Total Protein from Human Tumor Tissue: Stomach |
|
P1235248 |
Biochain |
1 mg |
EUR 320 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
 Total Protein from Human Diabetic Tissue: Stomach |
|
P1236248Dia |
Biochain |
1 mg |
EUR 461 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
 Total Protein from Mouse Normal Tissue: Stomach |
|
P1334248 |
Biochain |
1 mg |
EUR 214 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
 Membrane Protein from Human Tumor Tissue: Stomach |
|
P3235248 |
Biochain |
0.1 mg |
EUR 408 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
 Total RNA from Lupus: Stomach |
|
R1236248Lup-50 |
Biochain |
50 ug |
EUR 351 |
|
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
) Stomach Tissue Slide (Malignant Tumor) |
|
10-807-10um |
ProSci |
10 um |
EUR 201.5 |
Stomach Tissue Slide (Malignant Tumor) |
|
10-807-4um |
ProSci |
4 um |
EUR 180.5 |
) Stomach Tissue Slide (mucinous Adenocarcinoma) |
|
10-810-10um |
ProSci |
10 um |
EUR 201.5 |
Stomach Tissue Slide (mucinous Adenocarcinoma) |
|
10-810-4um |
ProSci |
4 um |
EUR 180.5 |
Stomach Tissue Slide (Malignant Tumor) |
|
RF10002-05-10um |
ProSci |
10 um |
EUR 201.5 |
Stomach Tissue Slide (Malignant Tumor) |
|
RF10002-05-4um |
ProSci |
4 um |
EUR 180.5 |
) Stomach Lysate (0 Day Old) |
|
1415-0 |
ProSci |
0.1 mg |
EUR 191 |
|
Description: Stomach tissue lysate (0 Day Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
) Stomach Lysate (14 Day Old) |
|
1415-14 |
ProSci |
0.1 mg |
EUR 191 |
|
Description: Stomach tissue lysate (14 Day Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
) Stomach Lysate (7 Day Old) |
|
1415-7 |
ProSci |
0.1 mg |
EUR 191 |
|
Description: Stomach tissue lysate (7 Day Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
 ELISA Kit) Chicken Anti-Alanyl-tRNA Synthetase, Cytoplasmic Antibody (AARS) ELISA Kit |
|
abx355511-96tests |
Abbexa |
96 tests |
EUR 825 |
|
|
 Total Protein from Human Adult Normal Tissue: Stomach |
|
P1234248 |
Biochain |
1 mg |
EUR 214 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
 Membrane Protein from Human Adult Normal Tissue: Stomach |
|
P3234248 |
Biochain |
0.1 mg |
EUR 285 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
 Human Stomach OptiTDS: Tissue Dissociation System |
|
4-28104 |
CHI Scientific |
1 Kit |
Ask for price |
 Mouse Stomach OptiTDS: Tissue Dissociation System |
|
4-28202 |
CHI Scientific |
1 Kit |
Ask for price |
 Rat Stomach OptiTDS: Tissue Dissociation System |
|
4-28292 |
CHI Scientific |
1 Kit |
Ask for price |
 Human Cancer PrimaCell16: Stomach Tumor Cells |
|
2-96039 |
CHI Scientific |
1 Kit |
Ask for price |
 cDNA from Human Tumor Tissue: Stomach |
|
C1235248 |
Biochain |
40 reactions |
EUR 540 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 cDNA from Human Diabetic Tissue: Stomach |
|
C1236248Dia |
Biochain |
40 reactions |
EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 cDNA from Mouse Normal Tissue: Stomach |
|
C1334248 |
Biochain |
40 reactions |
EUR 376 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 cDNA from Rat Normal Tissue: Stomach |
|
C1434248 |
Biochain |
40 reactions |
EUR 376 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 cDNA from Dog Normal Tissue: Stomach |
|
C1734248 |
Biochain |
40 reactions |
EUR 376 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Total RNA from Diabetic Disease: Stomach |
|
R1236248Dia-50 |
Biochain |
50 ug |
EUR 351 |
|
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Frozen Tissue Section - Human Tumor: Stomach |
|
T1235248 |
Biochain |
5 slides |
EUR 338 |
|
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
 Matched Pair Tissue Slides - Stomach Tissue |
|
10-800-MPPT |
ProSci |
1 pack |
EUR 201.5 |
|
Description: The Matched Pair Paraffin Tissue (MPPT) slides are designed for identifying tumor-specific/metastasis genes or proteins. Slices from normal and malignant tissues are mounted on each MPPT slide which can then be treated as a single histological slide for H&E staining, immunohistochemistry, or in situ hybridization. This format allows a rapid analysis of protein expression and localization across normal and cancerous tissue. |
 Multiple Species Tissue Slides - Stomach Tissue |
|
10-800-MSTA |
ProSci |
1 pack |
EUR 201.5 |
|
Description: The Multiple Species Tissue Array (MSTA) slides were designed to study protein expression patterns in different cells and tissues from multiple species. Tissue slices from three different species are mounted on each MSTA slide which can then be treated as a single histological slide for H&E staining, immunohistochemistry, or in situ hybridization. This format allows a rapid analysis of protein expression and localization across different species. MSTA slides can also be used to quickly determine the species reactivity of a given antibody. |
 Cytoplasmic Protein NCK1 Recombinant Protein |
|
92-102 |
ProSci |
0.05 mg |
EUR 490.25 |
|
Description: Cytoplasmic Protein NCK1 (NCK1) is a cytoplasmic protein that contains one SH2 domain and three SH3 domains. NCK1 is a member of the adapter family, which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. NCK1 maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. NCK1 plays a role in the DNA damage response, but not in the detection of the damage by ATM/ATR. It is also involved in transducing signals from receptor tyrosine kinases to downstream signal recipients, such as ELK1-dependent transcriptional activation in response to activated Ras signaling. |
 Total Protein from Human Adult Normal Tissue: Stomach: Cardia |
|
P1234250 |
Biochain |
1 mg |
EUR 214 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
 Total Protein from Human Adult Normal Tissue: Stomach: Corpus |
|
P1234251 |
Biochain |
1 mg |
EUR 214 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
 Total Protein from Human Adult Normal Tissue: Stomach: Fundus |
|
P1234252 |
Biochain |
1 mg |
EUR 214 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
 Total Protein from Human Adult Normal Tissue: Stomach: Pylorus |
|
P1234253 |
Biochain |
1 mg |
EUR 214 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
 Membrane Protein from Human Adult Normal Tissue: Stomach: Cardia |
|
P3234250 |
Biochain |
0.1 mg |
EUR 285 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
 Membrane Protein from Human Adult Normal Tissue: Stomach: Corpus |
|
P3234251 |
Biochain |
0.1 mg |
EUR 285 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
 Membrane Protein from Human Adult Normal Tissue: Stomach: Fundus |
|
P3234252 |
Biochain |
0.1 mg |
EUR 285 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
 Membrane Protein from Human Adult Normal Tissue: Stomach: Pylorus |
|
P3234253 |
Biochain |
0.1 mg |
EUR 285 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
) Cytoplasmic protein NCK2 Antibody (FITC) |
|
20-abx106742 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
|
) Cytoplasmic protein NCK2 Antibody (HRP) |
|
20-abx108161 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
|
) Cytoplasmic protein NCK2 Antibody (Biotin) |
|
20-abx105323 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
|
 Antibody) Cytoplasmic Protein NCK2 (NCK2) Antibody |
|
20-abx109274 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
|
 Cytoplasmic protein NCK2 Polyclonal Antibody |
|
42268-100ul |
SAB |
100ul |
EUR 333 |
 DR6 Cytoplasmic Domain Recombinant Protein |
|
95-119 |
ProSci |
0.05 mg |
EUR 322.25 |
|
Description: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer (reviewed in 1). Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNF-R1 and Fas. Several death receptors including DR3, DR4, DR5, and DR6 have been identified. Like TNF-R1, DR6 (also known as TNFRSF21) interacts with death domain containing adapter molecule TRADD. Overexpression of DR6 induces apoptosis and activates NF-kB and JNK. DR6 is widely expressed in human tissues and cell lines (2). Recently, DR6 was found to interact with an amino-terminal fragment of the Beta-amyloid protein (APP) in neurons, activating a caspase 6-dependent apoptotic event leading to axonal degeneration and pruning (3). The doublet observed in the SDS-PAGE is likely due to incomplete removal of the signal sequence. |
Changes within the loci Gga_rs16057130 and Gga_rs15788101 within the B5 line of the Smena 8 chickens are most probably related to synthetic choice of broiler productiveness traits, which may subsequently result in fixation of alleles at these loci. Artificial breeding of chickens results in degradation of the variability of genes encoding components of the immune system, which may trigger a lower in resistance to numerous ailments. The examine of the detrimental affect of choice of financial traits on immunity ought to present means to mitigate detrimental penalties and assist discover methods to acquire disease-resistant animals.
