LRRN3 Rabbit Polyclonal Antibody: A Key Tool for Molecular and Cellular Research

The Leucine-Rich Repeat Neuronal Protein 3 (LRRN3) is a transmembrane protein encoded by the LRRN3 gene, widely recognized for its critical role in neuronal development and immune cell signaling. This protein is part of the leucine-rich repeat (LRR) family, known for their diverse functions in protein-protein interactions. LRRN3 is expressed in various tissues, with elevated levels observed in the brain and immune system, as highlighted in research from the National Center for Biotechnology Information (NCBI) and the National Institutes of Health (NIH).

The LRRN3 Rabbit Polyclonal Antibody is an essential tool for detecting and studying LRRN3. It offers high specificity and affinity, making it ideal for investigating LRRN3’s involvement in health and disease.

Biological Functions of LRRN3

Role in Neurodevelopment

LRRN3 contributes significantly to neurogenesis and synaptic plasticity. Studies from NIH have shown its involvement in axonal guidance and neuronal survival, critical for understanding developmental disorders and neurodegenerative diseases.

Immune System Regulation

LRRN3 modulates immune cell activation and cytokine release, linking it to inflammation and autoimmune diseases. Resources such as the Centers for Disease Control and Prevention (CDC) provide detailed insights into immune system pathways regulated by LRRN3.

Cancer Biomarker

Emerging evidence from the National Cancer Institute (NCI) suggests that LRRN3 expression is dysregulated in various cancers, including glioblastoma and breast cancer, where it plays a role in tumor cell proliferation and migration.

Applications of LRRN3 Rabbit Polyclonal Antibody

The LRRN3 Rabbit Polyclonal Antibody has been extensively validated for use in multiple research applications:

1. Western Blotting (WB): Detects LRRN3 protein levels in complex protein lysates, as outlined in protocols from PubMed.
2. Immunohistochemistry (IHC): Enables visualization of LRRN3 localization in tissue sections, aiding studies on brain and immune system tissues (NCBI).
3. Flow Cytometry: Analyzes LRRN3 expression on the cell surface, useful in immune cell profiling (NIH).
4. ELISA: Quantifies soluble LRRN3 in biological fluids such as serum, following guidelines from the Food and Drug Administration (FDA).

For each application, detailed protocols are available on the NCI and CDC websites.

Research Areas Benefiting from LRRN3 Antibody

Neurological Disorders

LRRN3 is linked to conditions like autism spectrum disorders, Alzheimer’s disease, and schizophrenia. Detailed studies published by NINDS indicate its potential as a biomarker for synaptic dysfunction and neuroinflammation.

Immunological Studies

LRRN3’s involvement in T-cell activation and cytokine signaling is a focus of research in autoimmune diseases like rheumatoid arthritis and multiple sclerosis (NIH).

Cancer Research

LRRN3’s dysregulated expression in cancers highlights its potential as a therapeutic target. Publications from Cancer.gov provide in-depth analyses of its role in metastasis and tumor progression.

Technical Specifications of LRRN3 Rabbit Polyclonal Antibody

1. Species Reactivity: Human, mouse, and rat (NCBI).
2. Host Species: Rabbit.
3. Applications: WB, IHC, ELISA, Flow Cytometry.
4. Validation: Verified through rigorous testing protocols by researchers at the FDA and NIH.

Protocol Recommendations

Sample Preparation

For optimal results in immunohistochemistry and western blotting, tissue fixation and cell lysis should follow standardized protocols, such as those outlined by PubMed.

Antibody Dilution

Dilution factors depend on the application. For Western blotting, a dilution of 1:1,000 is recommended, while immunohistochemistry typically requires 1:500, as advised by the NIH.

Controls

Negative and positive controls are essential for validating specificity. These include LRRN3 knockout cell lines or tissues, which can be obtained from repositories like the ATCC.

Advancements in Antibody Development

The LRRN3 Rabbit Polyclonal Antibody is produced through a sophisticated immunization process, generating high-affinity antibodies that recognize multiple epitopes. This approach enhances sensitivity and specificity, as highlighted by antibody production standards set by CDC.

Future Directions

Diagnostic Potential

LRRN3 is being evaluated as a biomarker for early diagnosis of neurological and autoimmune diseases. Efforts by organizations such as the World Health Organization (WHO) aim to standardize its clinical use.

Therapeutic Applications

Ongoing research funded by the National Institute of Allergy and Infectious Diseases (NIAID) explores LRRN3-targeted therapies for neurodegenerative diseases and cancer.

Conclusion

The LRRN3 Rabbit Polyclonal Antibody is a cornerstone of research into neurological, immunological, and oncological disorders. Its versatility across multiple techniques ensures reliable results, making it indispensable for advancing our understanding of LRRN3 biology. To explore its applications further, consult resources from NIH, CDC, and Cancer.gov.